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Histo MLT 1215

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1 Histo MLT 1215 on Fri Apr 02, 2010 8:36 pm

ZENKERS ACETIC:
• Ingredients in Zenkers are:

STOCK SOLUTION
1.mercuric chloride – protein coagulant – harsh denaturing effect on proteins (oxidizer)

2.potassium dichromate – protein coagulant – functions as a NONCOAGULATING FIXING AGENT –which has a pH of 3.8 or greater – gentle denaturing effect on protein

3.sodium sulphate – indifferent salt – aids in penetration of the fixative

4.distilled water – diluent

5.glacial acetic acid – add only prior to use – counteracts shrinkage

HELLY’S – (ZENKER’S FORMOL):

STOCK SOLUTION

1.mercuric chloride – protein coagulant – has a harsh denaturing effect on proteins (oxidizer)

2.potassium dichromate – protein coagulant – functions as a NONCOAGULATING FIXING AGENT –which has a pH of 3.8 or greater – gentle denaturing effect on protein

3.sodium sulphate – indifferent salt – aids in penetration of the fixative

4.distilled water – diluent

5.formaldehyde – add only prior to use – (reducer)

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